Journal: iScience
Article Title: Genetic dissection of the role of Piga and Pgap2 in the embryonic mouse brain
doi: 10.1016/j.isci.2026.116299
Figure Lengend Snippet: Late onset hypomyelination in Olig2 ; Piga mutant brains (A) Percent genotype ratios of wildtype, Olig2 Cre/wt ; Piga flox/X female and Olig2 Cre/wt ; Piga flox/Y male survival to P21 ( n = 96, Chi-square p = 0.609). Immunostaining of MBP (B–D), CONTACTIN1 (E–G), SOX10 (H–J) and PDGFRα (K–M) in wild type (B, E, H, and K) Olig2 Cre/wt ; Piga flox/X female (C, F, I, and L) and Olig2 Cre/wt ; Piga flox/Y male (D, G, J, and M) animals. MBP and CONTACTIN1 data are from P96 (scale bar, 500 μm) while SOX10 and PDGFRα are from P21 (scale bar, 25 μm) ( n = 4 animals per genotype). Quantification of SOX10 (N, n = 3 animals per genotype, ANOVA p = 0.170), PGDFRα (O, n = 4, ANOVA p = 0.564) positive cells and the PDGFRα/SOX10 ratio (P, n = 3, ANOVA p = 0.430). Data represent individual values and the mean ± s.e.m. White arrows indicate SOX10 and PDGFRα positive cells.
Article Snippet: B6.129- Olig2 tm1.1(cre)Wdr/J or Olig2-Cre (IMSR_JAX:025567, B6.129S2- Emx1 tm1(cre)Krj/J ( Emx1-Cre , IMSR_JAX:#005628 animals were genotyped using Jackson Lab’s recommended general Cre genotyping.
Techniques: Mutagenesis, Immunostaining